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Our data shows that the adult algorithm failed to identify the bone area in small bones half of the time. The pediatric algorithm increased sensitivity in finding lower density small bones; however, in the axial region and large bones it often defined lean mass. The Discovery algorithm identified more of the bone area in small bones than the adult algorithm without the tendency to identify lean tissue as bone in the axial and large bones. Conclusions: The Discovery software was more accurate in identifying bone area than either the adult or pediatric options. Disclosures: B.

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M003 Osteoporosis Resulting From Deficient Acquisition of Peak Bone Mass: the SAMP6 Mouse Model. Van Herck*, R.

K.U.Leuven, Leuven, Belgium. The SAMP6 (P6) mouse model is characterized by spontaneous fractures and is considered an animal model of senile osteoporosis. In line with human findings in hip fracture patients, P6 mice show thinner but wider bone cortices. Presently, it is unclear when and how these structural abnormalities of the bone cortex develop.

This in vivo study was designed to monitor acquisition of peak bone mass longitudinally in (male and female) P6 and control SAMR1 (R1) mice. To this end, serial peripheral computed tomography (pQCT) and dual‐energy X‐ray absorptiometry (DEXA) measurements were performed from 4 weeks till 20 weeks of age. At week 4 also urinary deoxypyridino‐line (DPD) and serum osteocalcin (oc) levels were determined.

Peak bone mass acquisition in the tibia of R1 was characterized by greater cortical thickness in males than females. Male R1 showed 39% periosteal expansion, compared to only 18% at the endocortical site; females had less periosteal (24%) and no endocortical expansion. In both genders, total‐body bone mineral content (tBMC) and polar moment of inertia (pCSMI) correlated with body weight (r 2= 0.59 and r 2=0.74 respectively, p. Knee flexion and extension strength correlated with hip BMD (r 2=0.22−0.27, p 85 th percentile) and 94 normal weight (BMI ≤ 85 th percentile) subjects, aged 4–20 yr.

Proximal femur, dual energy x‐ray absorptiometry (DXA, Hologic QDR 4500) scans were analyzed at the femoral shaft (FS) and narrow neck (NN) by the Hip Structure Analysis Program. Subperiosteal width and indices of axial and bending strength (bone cross sectional area ‐ CSA ‐ and section modulus ‐ Z) were measured from bone mass profiles and cortical thickness was estimated using models. Log transformed regressions were used to determine percent differences in bone parameters in overweight compared with controls. After adjusting for height, maturation and gender, Z was 11 [95% C.I. 5, 19] and 13 [7, 20]% higher at the FS and NN, respectively, in overweight subjects (p 0.22) after adjusting for lean mass, with the exception of NN subperiosteal width (+3%, [0, 6], p = 0.04).

Fat mass did not contribute significantly to any model. In summary, proximal femur bone strength in overweight children is appropriately adapted to their lean body mass and height, but greater weight in the form of fat mass does not have an independent effect on bone bending strength. These geometric adaptations are consistent with the mechanostat hypothesis where bone strength adapts to skeletal loads dominated by muscle forces, but not to static loads represented by body weight.